Related Research on Traditional Chinese Medicine Syndromes and TNM Staging and Tumor Antigen in Primary Lung Cancer / 世界科学技术-中医药现代化

This article was aimed to study the relevance on traditional Chinese medicine (TCM) syndromes and TNM staging and tumor antigen in primary lung cancer, in order to provide theoretical supports for TCM prevention and treatment as well as medication guidance of primary lung cancer. Statistical data from 388 lung cancer patients were analyzed according to the relevance TCM syndromes, in order to find the relation between syndrome distribution of lung cancer and TNM staging and tumor antigens. The results showed that in TNM staging, the incidence of lung-yin deficiency syndrome in I stage was apparently higher than that in IV stage; the incidence of spleen-qi deficiency syndrome in IV stage was apparently higher than that in I stage; and there was no obvious difference in lung-qi deficiency syndrome, stasis obstructing lung collateral syndrome or lung-yin deficiency with fire-excess. The proportion of CEA in lung-yin deficiency with fire-excess syndrome was significantly higher than that in lung-yin deficiency syndrome, lung-qi deficiency syndrome, and stasis obstructing lung collateral syndrome. The proportion of abnormal increasing of NSE in lung-yin deficiency with fire-excess syndrome was significantly higher than other syndromes. The proportion of abnormal increasing of CYFRA21-1 in lung-yin deficiency with fire-excess syndrome and stasis obstructing lung collateral syndrome was significantly higher than that in lung-qi deficiency syndrome. It was concluded that there were certain relevance between TCM syndromes and TNM staging in lung cancer. Lung-yin deficiency syndrome, which existed in all stages of lung cancer, was the most obvious in the early stage. Spleen-qi deficiency syndrome was commonly seen in the advanced stage of lung cancer. There were certain relevance between different TCM syndromes and the abnormal increasing of tumor antigens including CEA, NSE and CYFRA21-1.

Relevance Research between Traditional Chinese Medicine Syndromes and Chemotherapy and Targeted Therapy on Primary Lung Cancer / 世界科学技术-中医药现代化

This article was aimed to study the relevance between traditional Chinese medicine ( TCM ) syndromes and the treatment of chemotherapy and targeted therapy , in order to provide theoretical support for TCM syndromes of lung cancer in the clinical application . Uniform TCM syndromes questionnaire was used in the TCM syndrome differentiation among lung cancer patients from the Oncology Department of Longhua Hospital, Shanghai University of Traditional Chinese Medicine and TCM Department of Shanghai Pulmonary Hospital. The analysis was made on correlation between distribution rules of TCM syndromes and chemotherapy and targeted therapy . The results showed that among primary lung cancer patients , the most TCM syndromes were syndrome of lung-yin deficiency , lung-q i deficiency , blood stasis in lung collaterals , spleen-q i deficiency , lung-yin deficiency with fire excess . There were certain correlation in TCM syndromes and different TCM therapeutic methods , in which targeted therapy had great significant effect on syndrome of lung-yin deficiency ( P < 0 . 05 ) , after targeted therapy syndrome of lung-yin deficiency increased obviously . It was concluded that there were some regulations in TCM syndromes of lung cancer which take syndrome of lung-yin deficiency, lung-qi deficiency, blood stasis in lung collaterals, spleen-qi deficiency, lung-yin deficiency with fire excess. There were certain correlation in TCM syndromes and different TCM therapeutic methods , after targeted therapy syndrome of lung-yin deficiency increased obviously .

Expression and purification of recombinant metallothionein of Eriocheir sinensis in E. coli / 生物医学工程学杂志

Metallothioneins (MT) are potential candidates for medicine development and application. For the purpose of expressing recombinant MT in E. coli, a crab MT cDNA cloned into pGEM-T was subcloned into pET-GST and then transformed into Escherichia Coli BL21. The fusion protein was proved to be expressed in both soluble and insoluble form by SDS-PAGE and western blot. Since metallothionein chelate metal ions, which may effects the physiological process of E. coli, caused the production of recombinant protein was lower than expected. Optimization of the ions content in the culture medium improved expression. The protein was purified by Zn2+ affinity chromatography, and rinsed off with high imidazole (1.5 M) which was the result of MT chelating instead of His-tag. This fusion protein laid a foundation of further study on the structural and functional biology of metallothionein.